Coding
Part:BBa_K3582012:Design
Designed by: Avadhoot Jadhav Group: iGEM20_IISER-Pune-India (2020-10-15)
Inhibitory Peptide sequence 1 for CD36-PfEMP CIDRa Domain Interaction
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The peptide was designed by mutating only one amino acid to ensure that the folding of the overall protein structure is not significantly disturbed.
Source
The sequence is obtained by performing saturated mutagenesis on the wild type interacting sequence of the host protein.
References
1] The structural basis for CD36 binding by the malaria parasite. Fu-Lien Hsieh1, Louise Turner2, Jani Reddy Bolla3, Carol V. Robinson3, Thomas Lavstsen2 & Matthew K. Higgins1